Rotationally acquired four-dimensional optical coherence tomography of embryonic chick hearts using retrospective gating on the common central A-scan

verfasst von

Christoph M. Happel, Jan Thommes, Lars Thrane, Jörg Männer, Tobias Ortmaier, Bodo Heimann, Talat Mesud Yelbuz

Abstract

We introduce a new method of rotational image acquisition for four-dimensional (4D) optical coherence tomography (OCT) of beating embryonic chick hearts. The rotational axis and the central A-scan of the OCT are identical. An out-of-phase image sequence covering multiple heartbeats is acquired at every angle of an incremental rotation of the deflection mirrors of the OCT system. Image acquisition is accomplished after a rotation of 180°. Comparison of a displayed live M-mode of the central A-scan with a reference M-mode allows instant detection of translational movements of the embryo. For calculation of 4D data sets, we apply an image-based retrospective gating algorithm using the phase information of the common central A-scan present in all acquired images. This leads to cylindrical three-dimensional data sets for every time step of the cardiac cycle that can be used for 4D visualization. We demonstrate this approach and provide a video of a beating Hamburger and Hamilton stage 16 embryonic chick heart generated from a 4D OCT data set using rotational image acquisition.

Organisationseinheit(en)

Institut für Mechatronische Systeme

Externe Organisation(en)

Medizinische Hochschule Hannover (MHH)
Technical University of Denmark
Georg-August-Universität Göttingen

Typ

Artikel

Journal

Journal of biomedical optics

Band

16

ISSN

1083-3668

Publikationsdatum

09.2011

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Elektronische, optische und magnetische Materialien, Biomaterialien, Atom- und Molekularphysik sowie Optik, Biomedizintechnik

Elektronische Version(en)

https://doi.org/10.1117/1.3622491 (Zugang: Offen)