Construction and Establishment of a New Environmental Chamber to Study Real-Time Cardiac Development

verfasst von

Gülay Orhan, Stephan Baron, Kambiz Norozi, Jörg Männer, Oliver Hornung, Holger Blume, Judith Misske, Bodo Heimann, Armin Wessel, T. Mesud Yelbuz

Abstract

Heart development, especially the critical phase of cardiac looping, is a complex and intricate process that has not yet been visualized "live" over long periods of time. We have constructed and established a new environmental incubator chamber that provides stable conditions for embryonic development with regard to temperature, humidity, and oxygen levels. We have integrated a video microscope in the chamber to visualize the developing heart in real time and present the first "live" recordings of a chick embryo in shell-less culture acquired over a period of 2 days. The time-lapse images we show depict a significant time window that covers the most critical and typical morphogenetic events during normal cardiac looping. Our system is of interest to researchers in the field of embryogenesis, as it can be adapted to a variety of animal models for organogenesis studies including heart and limb development.

Organisationseinheit(en)

Institut für Mechatronische Systeme
Mechatronik-Zentrum Hannover

Externe Organisation(en)

Medizinische Hochschule Hannover (MHH)
Georg-August-Universität Göttingen

Typ

Artikel

Journal

Microscopy and Microanalysis

Band

13

Seiten

204-210

Anzahl der Seiten

7

ISSN

1431-9276

Publikationsdatum

09.05.2007

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Instrumentierung

Elektronische Version(en)

https://doi.org/10.1017/S1431927607070390 (Zugang: Geschlossen)
https://doi.org/10.15488/2669 (Zugang: Offen)